P-090 The in vitro motility characteristics of human sperm collected by microfluidic device

Abstract Study question Do the in vitro motility characteristics of sperm collected using a microfluidic device (ZyMot) differ from those by density gradient centrifugation (DGC)? Summary answer ZyMot-collected had longer-lasting than DGC, but head beat-cross frequency was inferior due to absence washing vitro. What is known already ZyMot (DxNow, Inc., USA) can sort highly motile with low reactive oxygen species (ROS), and DNA damage, without centrifugation. In IUI cycles, although total number lower that it reported pregnancy rate cycles higher DGC cycles. Washing medium essential for capacitation; however, protocol devices lacks this procedure. The aim study clarify sperm. We also examined how affected motility. design, size, duration specimens were provided patients who underwent semen analysis between October 2022 January 2023, written consent. used 12 samples volume 2.5 ml or more, concentration 4 million/ml more. same donor divided into three groups (DGC, ZyMot, wash). Dunnett’s test performed group as control group. Participants/materials, setting, methods group, 2-layer (400G, 20 min) performed, followed 5 min). according manufacturer’s protocol. wash centrifugally washed (300G, All cultured at 30 °C until analysis. Sperm analyzed computer-assisted system (SMAS) after 0, 4, 24 h preparation. Main results role chance There no significant difference two compared 0 4h On other hand, tended be (53.3%, p = 0.0787), significantly (85.4%, < 0.001) (33.5%), 24h straight-line velocity, curvilinear average speed did not 4h. parameters culturing. Regarding movement, there amplitude lateral displacement culture. contrast, (9.18±0.31 Hz vs 8.71±0.49 Hz, 0.0409). Both movement Limitations, reasons caution chose high because we experimental groups. Therefore, unclear whether our findings are applicable poor parameters. Wider implications may advantageous maintains sustainable extended periods. Alternately, unfavorable conventional IVF which marker capacitation. However, disadvantage overcome washing. Trial registration